Comparison Study of Binding Kinetics on OpenSPR, Biacore and IBIS

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چکیده

OpenSPRTM is a powerful instrument providing indepth label-free binding kinetics for a variety of different molecular interactions. With any new technology, it is important to compare the performance of various tools and techniques to establish their consistency and accuracy. In this study, binding kinetic results of protein-protein interactions are compared between the OpenSPRTM, BiacoreTM and IBISTM MX96 surface plasmon resonance instruments. The biomolecular system and conditions were developed by Dr. Olan Dolezal from CSIRO. The kon, koff, and KD of two different ligands (M1 and M2) to one analyte are determined and compared.

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تاریخ انتشار 2016